Research: Antibiotic Sensitivity Pattern of Salmonella Species Isolated from Blood Culture

Mar 08 2019

INTRODUCTION: Typhoid fever occurs in all parts of the world where water supplies and sanitation are sub-standard. The disease in now uncommon in developed countries where most of the cases that occurs are either acquired aboard or imported by immigrants.

OBJECTIVE: The aim of this study is to determine the antibiotic sensitivity pattern of Salmonella species isolated from blood culture.

METHODS: A retrospective study was conducted in the period Oct 2002 to Sept 2003 in Kathmandu Medical College Teaching Hospital, Sinamangal Kathmandu. Blood sample for culture were collected by vein puncture with disposable syringe and immediately inoculated into brain heart infusion broth and incubated at 37C for 24 hours. After incubation sub-culture were done two times on blood agar and Salmonella -Shigella agar. Positive cultures were identified by standard microbiological technique. The entire isolates antibiotic sensitivity test was performed by disc diffusion method.

RESULTS: A total 4350 patients blood were cultured, suspecting for fever, out of that 390 (8.9655%) patients blood were found to be positive for Salmonella species. Males outnumbered females and age group (21-40) had the maximum number 185 (47.43%) followed by age group (0-20) 160 (41.025%) and age group (41-60) 45 (11.538%). In vitro antibiotic sensitivity test shows that ciprofloxacin is highly sensitive (98.718%) followed by ofloxacin (98.208%), Cephalexin (96.411%), chloramphenicol (95.898%), cotrimoxazol (94.103%) and ampicillin (87.18%).

CONCLUSION: The above study shows that prevalence of enteric fever was found to be high. Prevention of enteric fever is mainly by personal and domestic hygine. The provision of safe drinking water and proper disposal of faeces will implement as soon as possible in Kathmandu valley.


Journal of Nepal Health Research Council  01; 3(2):35-38.