Direct measurement of oxidative and nitrosative stress dynamics in Salmonella inside macrophages

Apr 16 2018

Real-time in vitro analysis of intrabacterial redox potential.


Salmonellosis is an infection with bacteria called Salmonella. Salmonella germs have been known to cause illness for over 100 years. They were discovered by an American scientist named Salmon, for whom they are named.

Most persons infected with Salmonella develop diarrhea, fever, and abdominal cramps 12 to 72 hours after infection. The illness usually lasts 4 to 7 days, and most persons recover without treatment. However, in some persons, the diarrhea may be so severe that the patient needs to be hospitalized. In these patients, the Salmonella infection may spread from the intestines to the blood stream, and then to other body sites and can cause death unless the person is treated promptly with antibiotics. The elderly, infants, and those with impaired immune systems are more likely to have a severe illness.


The upper left panel shows the fluorescent intensities (405 nm in blue and 488 nm in green) after a challenge with 2.5 mM H2O2. The upper right panel shows the pseudocolored ratio movie of the same challenge, and the lower panel shows the corresponding graph with the normalized 405/480 ratio values.


© 2015 National Academy of Sciences